PAPER: Recent Technology Advances Transforming Drug Discovery and Development
As demands for safe and efficacious pharmaceutical drugs grow, technological solutions will be of increasing interest that reduce barriers to clinical success rather than simply shifting bottlenecks and necessitating new compromises in the discovery and development process. Discussed here are: (1) Target discovery and validation – impact of nanofluidic real-time qPCR technologies; (2) Advances in early stage compound screening – impact of high throughput, microfluidic mass spectrometry, and (3) Incorporating ADME/Tox data earlier in drug discovery.. More...
PAPER: Toxicogenomics screening of small molecules using high-density, nanocapillary real-time PCR
We screened 625 compounds from a fully combinatorial library for their gene expression profiles in vitro over a selected toxicity panel of 56 genes. We used the novel qPCR real-time OpenArray technology that couples analytical performance with medium throughput ideal for such a sample-per-feature raito. Applying a hybrid clustering on the gene expression data, correlation was analyzed between molecular scaffold and biological fingerprint. More...
PAPER: Development and experimental validation of a predictive threshold cycle (Ct) equation for quantification of virulence and marker genes using high-throughput nanoliter PCR on OpenArrays
Development of quantitative PCR (QPCR) assays typically requires extensive screening within and across a given species to ensure specific detection and lucid identification among various pathogenic and nonpathogenic strains and to generate standard curves. To minimize screening requirements, multiple virulence and marker genes (VMGs) were targeted simultaneously to enhance reliability, and a predictive threshold cycle (Ct) equation was developed to calculate starting copies based on experimental Ct. More…
POSTER: High-Throughput, Low Volume Endpoint And Real-Time PCR GMO Detection In The OpenArray® Platform
Efficient detection of genetically modified organisms is becoming increasingly important to comply with more restrictions and increased awareness in food safety. We demonstrate a novel highly parallel, nanofluidic system capable of performing above 3000 endpoint or real-time quantitative polymerase chain reactions, based on endpoint Taqman® or real-time SYBR® Green I detection, in a miniaturized through-hole array format. More…
POSTER: Nanoliter PCR-Based Applications For Agriculture Research
The application of marker/genetic selection technologies in plants and animals can potentially reduce the production time for new organisms with desirable traits. The expensive and time consuming nature of current technologies significantly limits the industrialization of this technique for large, high-throughput studies. The OpenArray™ platform, presented here, enables the researcher to interrogate a large number of nucleic acid samples against a large number of assays in a flexible, configurable format. More…
POSTER: OpenArray® platform: enabling high-throughput real-time qPCR applications in nanoliter volumes
BioTrove has developed the OpenArray™ platform based on through-hole technology, a broadly applicable nanoliter fluidics technology for parallel and low-volume solution phase reactions. OpenArray™ plates are coated with hydrophilic coatings on the interior of each through-hole and hydrophobic coatings on the exterior of the through-holes. This enables OpenArray™ plates to hold solutions in the open through-holes via capillary action. More…
POSTER: High throughput, nanofluidic real-time PCR analysis of gene expression in tumor samples
Understanding biological complexity arising from patterns of gene expression and gene function requires accurate and precise measurement of RNA levels across large numbers of genes simultaneously. We demonstrate a novel highly parallel, nanofluidic system capable of performing nearly 3000 real-time polymerase chain reactions (rt-PCR), based on SYBR® Green I detection, in a miniaturized through-hole array format. More…
PRESENTATION: SNP Analysis as a Tool for Microbial Subtyping
SNP-based genotyping promises rapid, high-throughput subtyping, improved speed and accuracy of outbreak clusters over traditional PFGE and lab and data analysis components that are both highly amenable to automation. The BioTrove OpenArray™ system provides a platform capable of high-throughput screening of candidate SNPs against a library of reference strains. More…
PAPER: Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays
Diagnostics and disease-management strategies require technologies to enable the simultaneous detection and quantification of a wide range of pathogenic microorganisms. Most multiplex, quantitative detection methods available suffer from compromises between the level of multiplexing, throughput and accuracy of quantification. Here, we demonstrate the efficacy of a novel, high-throughput, ligation-based assay for simultaneous quantitative detection of multiple plant pathogens. More…
POSTER: The OpenArray® Platform: Enabling High-Throughput SNP Genotyping and Real-Time PCR Applications in Nanoliter Volumes
BioTrove developed the OpenArray® platform based on through-hole technology, a broadly applicable nanoliter fluidics technology for parallel and low-volume solution phase reactions. OpenArray™ plates are coated with hydrophilic coatings on the interior of each through-hole and hydrophobic coatings on the exterior of the through-holes. More…
POSTER: High-Throughput, Low Volume Real-Time PCR Pathogen Detection in the OpenArray® platform
For decades efforts to detect and identify microorganisms from a variety of samples have been focused on amplification of microbial material (bacterial culture). Today, culture-based methods for pathogen detection are rapidly replaced by faster and more specific real-time PCR assays.
We demonstrate a novel highly parallel, nanofluidic system capable of performing over 3000 real-time polymerase chain reactions (Real-Time PCR), based on SYBR® Green I detection, in a miniaturized through-hole array format. More…
POSTER: Quantitative multiplex detection of pathogens using PRI-lock probes and ultra-high-throughput Q-PCR on OpenArray
Current technologies for multiplex, quantitative analyses frequently suffer from compromises between the level of multiplexing, through-put and accuracy of quantification. In general, for the detection of nucleic acids, microarrays provide a very high level of multiplexing, but less accurate quantification and usually low throughput. Recently, a conceptually new, ultra-high-throughput OpenArray® platform has become available for real-time PCR, capable of accommodating more than 3000 reactions per array. More…
POSTER: The OpenArray® Platform: Enabling High-Throughput SNP Genotyping Applications in Nanoliter Volumes
Single nucleotide polymorphism (SNP) genotyping has become increasingly important in both pharmaceutical and agricultural biotechnology as a means of identifying biologically important genetic variations. BioTrove has developed a SNP genotyping platform based on through-hole technology, a broadly applicable nanoliter fluidics technology for parallel and low-volume solution phase reactions. More…
PAPER: Nanoliter high throughput quantitative PCR
Understanding biological complexity arising from patterns of gene expression requires accurate and precise measurement of RNA levels across large numbers of genes simultaneously. Real time PCR (RT-PCR) in a microtiter plate is the preferred method for quantitative transcriptional analysis but scaling RT-PCR to higher throughputs in this fluidic format is intrinsically limited by cost and logistic considerations. Hybridization microarrays measure the transcription of many thousands of genes simultaneously yet are limited by low sensitivity, dynamic range, accuracy and sample throughput. The hybrid approach described here combines the superior accuracy, precision and dynamic range of RT-PCR with the parallelism of a microarray in an array of 3072 real time, 33 nl polymerase chain reactions (RT-PCRs) the size of a microscope slide. More…
POSTER: OpenArray® SNP Genotyping: A High-Throughput Nanoliter-Scale Platform for Detection of Single Nucleotide Polymorphisms
Single Nucleotide Polymorphism (SNP) genotyping has become increasingly important in both pharmaceutical and agricultural biotechnology as a means of identifying biologically important genetic variations. BioTrove has developed a high-throughput, nanoliter-scale genotyping platform that uses customers’ existing TaqMan® SNP assays and provides the ability to generate in excess of 200,000 genotypes per day. More…
PAPER: High throughput, nanoliter quantitative PCR
The recent completion of the human genome sequence has increased the need for high throughput quantitative transcription analysis. Quantitative PCR is an alternative to microarrays for accurate and precise expression analysis with single transcript copy sensitivity. A review of current research in miniaturized, high throughput qPCR suggests this technique will soon be a viable option to hybridization microarrays for large-scale genetic analyses. More…
