Validation of DNA array results is one of the most common uses of qPCR. Generally, with tedious plate-based systems researchers need to narrow their focus to keep projects to a manageable size. Using OpenArray to validate microarray hits allows you to cast a wider net; choosing genes that may be expressed at a lower level or that have a differential expression ratio less than 2-fold.
It has been shown that the OpenArray platform identifies differentially expressed genes below the sensitivity threshold of common chip and bead-based microarray platforms. The OpenArray Pathways panels allow you to use the OpenArray platform in a discovery mode; uncovering differentially expressed genes. Let's put the hypothesis back into research, and perform hypothesis-dependent expression profiling experiements.
Resources
(Please click on image or link to download)
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Pathways Product Profile.A complete and current listing of the Pathways panels available. Each Pathways panel contains 600+ bench-validated assays for genes involved in common disease processes or biological pathways. |
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Nanoliter high-throughput RT-qPCR: a statistical ....James M. Dixon, Mariusz Lubomirski, Dhammika Amaratunga, Tom B. Morrison, Colin J. H. Brenan and Sergey E. Ilyin. BioTechniques 46:ii-viii. |
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OpenArray DLP Product ProfileA summary of how customers use the OpenArray platform with TaqMan® chemistry. In addition, there is information about our workflow and analysis software. |
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Identification of Differentially Expressed Gene.....Andrew T. Bond, D. Roberts, E. Grigorenko, E. Ortenberg, K. Munnelly. PAG 2009. |